Spore Propagation

Closed system for spore production.

Sporebox for spore propagation

For the production of spores, in particular filamentous micro-organisms, Vogelbusch has developed a patented propagation system.

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Sporebox

The Sporebox consists of two parts, a supply unit, and a sterile compartment for spore pro­duction.

The supply unit contains all necessary equipment to ensure optimal cultivation condi­tions, such control of humidity, temperature, pressure and airflow rate as well as measuring instruments, recorder, ventilator and a com­pressor.

The production compartment has windows for optical inspection of the growth of the micro-organisms. Integrated gloves are used for all manipulations from the outside. Sterile ventilation is carried out by airflow through HEPA filter.

Production

The production compartment is equipped with 60 plates, each with a utilizable area of 1,260 cm², resulting in a total surface of 7.56 m².

For priming a blend of agar, molasses and mineral salts is sterilized and applied on the plates. Once the substrate is solidified, the surface is inoculated with the micro-organism.

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Harvest of spores

After a cultivation period under controlled conditions, the spores are cautiously dried, harvested and bottled. The bottled spores can be stored in a customary deep freezer at -20°C for extended periods.

Features

The advantages of spore propagation in a Vogelbusch Sporebox are:

  • Closed system - no outside contact from propagation and harvesting through to bottling
  • Easily controlled dosing - the amount of dry spores is determined by weight
  • Extended shelf life of spores
Yield
Example Aspergillus niger

Depending on the substrate (molasses) quality, for instance 0.8 – 1.4 kg spores of Aspergillus niger can be grown in each production cycle. The production cycle for Aspergillus niger lasts about 14 days: 5 days for cultivation, 5 days of drying, 4 days for harvesting, cleaning and preparations for the next cycle.

All in all some 25 kg of Aspergillus niger can be produced in one spore box per year.

Scope of supply

A basic unit consists of agar preparation, a Sporebox and the harvesting apparatus. Production capacity is extended with additional Spore Boxes while using the existing set of preparation and harvesting equipment.

The harvesting apparatus comprising a separator (cyclone), a vacuum cleaner, a safety filter for exhaust air and appropriate connections is included in delivery.

The agar preparation vessel (60 liter) can be supplied optionally.

Installation requirements
SPOREBOX
 Net weight  770 kg  
Dimensions 2200 x 1370 x 1790 mm
Power supply 240 V / 400 V
Cold water supply, outlet DN 20
Drain (sewer) DN 50
   
HARVESTING APPARATUS 
Power supply 400 V
   
AGAR PREPARATION VESSEL 
Power supply 240 V
Compressed air pressure cyclinder
Premises

The Sporebox has to be placed in an air-conditioned room with a temperature of 30°C ± 1°C at all points. A minimum of eight air exchanges per hour and the installation of coarse and fine filters in the ventilation ducts are recommended.

The air outlets should be mounted flush in the ceiling, just as the lighting fixtures. Ceiling, walls and floor should be as smooth as possible, fillets are recommended. The room should be without windows.

Reference projects including supply of sporeboxes

4 KGG
Citric Acid Plant in Kermanshah
KIMIA GHARB GOSTAR - Kermanshah, Iran

The plant of Kimia Gharb Gostar Industries Co. has a capacity of 36 tons per day and is the first citric acid plant set up in Iran. It is suitable for processing of raw sugar and sugar beet molasses. The process concept allows for a choice between production of either...

4-citrom0
Citric Acid Plant near Cairo
CITRO MISR - 10th of Ramadan City, Egypt

Raw sugar from local production was the substrate of choice for Citro Misr Co. of Egypt. This raw material required a new approach in the fermentation process to assure the economic feasibility of the plant. The spore cultivation was adapted and the fructose utilization was optimized for the substrate. Products include...

News

F.O. Licht's Sugar & Ethanol Asia
18 Dec 2017

March 6 - 8, 2018 in Bangkok, Thailand